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the 本发明提供的技术方案具体为一种BPC157肽的固相制备方法，包括以下步骤： The technical solution provided by the present invention is specifically a solid-phase preparation method of BPC157 peptide, comprising the following steps: 以三苯氯甲基树脂为合成树脂，包括4-甲基-三苯氯甲基树脂、4-甲氧基-三苯氯甲基树脂、2-氯-三苯氯甲基树脂或王氏树脂，以以上树脂中的任何一种为起始原料，按照固相合成的方法依次连接具有Fmoc保护的氨基酸，获的保护的BPC157肽树脂，其间依次脱去Fmoc保护基团，用DIC/HOBt或DIC/HOAt或BOP/HOBt或BOP/HOAt或HBTU/HOBt或HBTU/HOAt或HATU/HOBt或HATU/HOAt或HCTU/HOBt中的一种为缩合剂进行接肽反应，得保护的BPC157肽树脂后，同步进行脱侧链保护基团及切肽，获得BPC157肽粗品，并经C 18 或C 8 色谱柱进行分离纯化，获得所需的BPC157肽纯品。 Use trityl chloromethyl resin as synthetic resin, including 4-methyl-trityl chloromethyl resin, 4-methoxy-trityl chloromethyl resin, 2-chloro-trityl chloromethyl resin or Wang's Resin, with any one of the above resins as the starting material, according to the method of solid-phase synthesis, the amino acids with Fmoc protection are sequentially connected to obtain the protected BPC157 peptide resin, during which the Fmoc protection group is sequentially removed, and the DIC/HOBt Or one of DIC/HOAt or BOP/HOBt or BOP/HOAt or HBTU/HOBt or HBTU/HOAt or HATU/HOBt or HATU/HOAt or HCTU/HOBt is a condensing agent for peptide grafting reaction, and the protected BPC157 peptide resin Afterwards, remove the side chain protection group and cut the peptide simultaneously to obtain the crude product of BPC157 peptide, which is separated and purified by C 18 or C 8 chromatographic column to obtain the desired pure product of BPC157 peptide
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Capsule Count:60
The 5mg format provides researchers with a practical quantity of material suitable for multiple laboratory applications while supporting accurate measurement, handling, and experimental consistency
